X-Gal

Description:
X-Gal is a highly pure molecular biology grade chemical for colorimetric detection of b-galactosidase activity. It is used for “blue/white screening” of bacterial colonies to differ bacterial or phage clones after transformation. In combination with suitable bacterial cloning vectors, host strains, and IPTG, X-Gal provides an easy way to distinguish between positive and negative clones after transformation.

Quality:
Purity of >98% by HPLC. Tested in the blue/white colony screening experiment.

Note:
– Preparation of a 20 mg/ml stock solution in dimethylformamide or dimethylsulfoxide is recommended.
– Dimethylformamide dissolves some plastic materials. The direct addition of dimethylformamide containing solution to plastic Petri dishes should be avoided.

Storage:
at – 20°C in the dark; shipped at ambient temperature

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