The Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates.
Description:
The GF-1 Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. This kit uses the alkaline lysis-SDS method to lyse cells and release plasmid DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure plasmid DNA.
Features:
– Yields up to 20µg of DNA
– Multiple samples can be processed rapidly in less than 30 minutes
– No organic-based extraction required
– Highly pure plasmid DNA ready to use for routine molecular biology applications such as restriction
enzyme digestion, PCR, DNA sequencing, ligation, transformation, etc.
Application:
For high quality plasmid DNA isolation.
Kit components:
– GF-1 columns
– Collection tubes
– Lösung 1 (S1)*
– Lösung 2 (S2)*
– Neutralisationspuffer (Puffer NB)
– Waschpuffer (Konzentrat)*
– RNase A (DNase-frei)*
– Elutionspuffer
– Handbuch
*Please refer to Reconstitution of Solutions and Storage and Stability before using this kit.
Product | Description |
PL05 | Plasmid DNA Extraction Kit The Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. Pack size: 5 preps |
PL50 | Plasmid DNA Extraction Kit The Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. Pack size: 50 preps |
PL100 | Plasmid DNA Extraction Kit The Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. Pack size: 100 preps |
PL200 | Plasmid DNA Extraction Kit The Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. Pack size: 200 preps |
MSDS
References
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Hamidinejat, H., et al. (2015) Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. BIran Journal of Parasitology. 10(1), p.69-77.
Hesampour, A., et al. (2014) Comparison of Biochemical Properties of Recombinant Phytase Expression in the Favorable Methylotrophic Platforms of Pichia pastoris and Hansenula polymorpha. Progress in Biological Sciences. 4(1), p. 97 – 111.
Gul, R., et al. (2012) Expression and Sequence Characterization of Growth Hormone Binding Protein of Nili-Ravi Buffaloes (Bubalus bubalis). African Journal of Biotechnology. ProQuest. 11(57), p. 12103-12109
Gul, R., et al. (2012) Expression and Sequence Characterization of Growth Hormone Binding Protein of Nili-Ravi Buffaloes (Bubalus bubalis). African Journal of Biotechnology. ProQuest. 11(57), p. 12103-12109
Pongsilp, N., et al (2012) Genotypic Diversity among Rhizospheric Bacteria of Three Legumes Assessed by Cultivation-dependent and Cultivation-independent Techniques. World Journal of Microbiology and Biotechnology.ProQuest. 28, p. 615-626
Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011)Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114.
Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010) Isolation and Characterization of a Novel Thermophilic-organic Solvent Stable Lipase from Acinetobacter baylyi Applied Biochemistry and Biotechnology.