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COT I Human DNA (blocking reagent)

Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing

Description:
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
The product is prepared from male human placental DNA, exclusively.
The COT I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.

Concentration: > 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4

Quality control:
– Average fragments size: 50-300 bp;
– A260/A280 ration:  about 1.78;
– Amount of genomic (non-repetitive DNA): less than 2%.
COT I DNA and the raw material is tested for the absence of HIV1,2 RNA, HCV RNA, HBV DNA

Transportation: on blue ice

Storage: at -20°C for more than 12 months

 

ProductDescription
39001COT I Human DNA

Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing

500 µg

39005COT I Human DNA

Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing

5 mg

Pricelist

Datasheet Cot I human DNA

Stability Test Report

Material Safety Datasheet

References

Recommendation from a researcher:

For Southern blot hybridizations: add 50 μg of COT-1 human DNA (@ 10 μg/μL) to 50 μL of 20X SSC, 25 μL distilled water and 20 μL of a solution containing 0.1 M NaCl, 0.1 M Tris-HCl (pH 7.4). 0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe.

For in situ hybridizations: combine genomic probe with the proper amount of COT-1 human DNA such that the final concentration of COT-1 human DNA is 0.3 μg/μL for cosmid, plasmid, and lambda probes; or, at 1 μg/μL for Alu PCR probes.
Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 °C for 5 min. Incubate at 37 °C for at least 5 and up to 15 min.

Interesting links:

High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering.

Bao Paul; Frutos Anthony G; Greef Charles; Lahiri Joydeep; Muller Uwe; Peterson Todd C; Warden Laurence; Xie Xinying;
Anal Chem (2002) 74:1792-1799
Product usage: Superscript II RT was used to make labeled cDNA from a human polyA RNA sample (42C incubation temp). Cy3 or Cy5 labeled dCTP was used for these experiments. Remaining RNA was degraded by using a combination of RNase H (Invitrogen) and RNase A (USB). Array hybridization was per……


Isolation and characterization of the human cytochrome P450 CYP1B1 gene.

Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F;
J Biol Chem (1996) 271:28324-28354
Product usage: Cultures of the human squamous cell carcinoma line SCC12(c12c2) were plated at 2.5 × 106 to 5 × 106 cells/60-mm dish and grown to 70-90% confluence before transfection. The transient transfection was performed by a lipofection method in a serum-free medium with 2.5 µg of each pla……