Cot I DNA für: Mikroarray Anwendungen, komparative genomische Hybridisierung (CGH), Karyotyping , In-Situ Hybridisierung
Beschreibung:
Die Cot I Humane DNA wird ausschließlich aus Plazenten von Frauen gewonnen, die ein männliches, neugeborenes Kind ausgetragen haben. Spezielle, aufwendige Aufreinigungsverfahren reichern die DNA mit repetitiven Elementen an. Cot I Humane DNA Fraktion von GeneON enthält mindestens 98 % dieser repetitiven und schnell anlagernden Elemente.
Anwendungen:
Unterdrückung von Kreuzhybrisierungen menschlicher repetitiver DNA bei:
– Filter- oder Mikroarray Hybridisierungen
– In-situ-Hybridisierungen
– Single-Copy“-Gen Hybridisierungen
Konzentration: > 1,1 mg / ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4
Qualitätskontrolle:
Durchschnittliche Fragmentgröße: 50-300 bp
A260/A280: 1.78
Enthaltene genomische DNA (non-repetitive DNA): < 2%.
Alle Chargen sind garantiert getestet auf und frei von HIV1,2 RNA, HCV RNA und HBV DNA
Transport: mit Kühlakkus
Lagerung: bei – 20°C für mindestens 12 Monate
| Artikel | Beschreibung |
| 39001 | COT I Human DNA Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing 500 µg |
| 39005 | COT I Human DNA Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing 5 mg |
Datasheet Cot I human DNA
Stability Test Report
Material Safety Datasheet
Referenzen
Recommendation from a researcher:
For Southern blot hybridizations: add 50 μg of COT-1 human DNA (@ 10 μg/μL) to 50 μL of 20X SSC, 25 μL distilled water and 20 μL of a solution containing 0.1 M NaCl, 0.1 M Tris-HCl (pH 7.4). 0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe.
For in situ hybridizations: combine genomic probe with the proper amount of COT-1 human DNA such that the final concentration of COT-1 human DNA is 0.3 μg/μL for cosmid, plasmid, and lambda probes; or, at 1 μg/μL for Alu PCR probes.
Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 °C for 5 min. Incubate at 37 °C for at least 5 and up to 15 min.
Interesting links:
High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering.
Bao Paul; Frutos Anthony G; Greef Charles; Lahiri Joydeep; Muller Uwe; Peterson Todd C; Warden Laurence; Xie Xinying;
Anal Chem (2002) 74:1792-1799
Product usage: Superscript II RT was used to make labeled cDNA from a human polyA RNA sample (42C incubation temp). Cy3 or Cy5 labeled dCTP was used for these experiments. Remaining RNA was degraded by using a combination of RNase H (Invitrogen) and RNase A (USB). Array hybridization was per……
Isolation and characterization of the human cytochrome P450 CYP1B1 gene.
Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F;
J Biol Chem (1996) 271:28324-28354
Product usage: Cultures of the human squamous cell carcinoma line SCC12(c12c2) were plated at 2.5 × 106 to 5 × 106 cells/60-mm dish and grown to 70-90% confluence before transfection. The transient transfection was performed by a lipofection method in a serum-free medium with 2.5 µg of each pla……