Seraglob by Bioswisstec AG info@seraglob.com +41 52 620 33 44 | Fax +41 52 620 33 45

seraglob

Ambi Gel/PCR Purification Kit

AmbiClean Kit (Gel & PCR) is a system designed for DNA recovery from agorose gel and rapid PCR clean-up of DNA bands ranging from 100bp to 20kb.

Description
The GF-1 AmbiClean Kit (Gel & PCR) contains special buffers to provide the correct salt concentration and pH for efficient recovery (80 – 90%) of DNA from both PCR product and agarose gel from both TAE or TBE buffer.
This “clean up” – kit uses a specially treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. It applies the principle of a mini-column spin technology and is well suited for the removal of agarose, excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after restriction enzyme treatment and dephosporylation and residual dye and ethidium bromide.
This extraction kit also allows for concentration of DNA, changing of buffers and desalting. High recovery of pure DNA obtained is ready-to-use in many routine molecular biology applications such as restriction enzyme digestion, radioactive/fluorescence DNA sequencing, PCR, ligation and transformation, probe preparations and other manipulations.

Kit components:
– GF-1 Columns
– Collection tubes
– DNA Binding Buffer (Buffer DB)
– Wash Buffer (concentrate)*
– Elution Buffer
– Handbook

* Please refer to Reconstitution of Solutions and Storage and Stability before using this kit.

ProductDescription
GV05Ambi Clean Gel/PCR DNA Extraction Kit

AmbiClean Kit (Gel & PCR) is a system designed for DNA recovery from agorose gel and rapid PCR clean-up of DNA bands ranging from 100bp to 20kb.

Pack size: 5 preps

GV50Ambi Clean Gel/PCR DNA Extraction Kit

AmbiClean Kit (Gel & PCR) is a system designed for DNA recovery from agorose gel and rapid PCR clean-up of DNA bands ranging from 100bp to 20kb.

Pack size: 50 preps

GV100Ambi Clean Gel/PCR DNA Extraction Kit

AmbiClean Kit (Gel & PCR) is a system designed for DNA recovery from agorose gel and rapid PCR clean-up of DNA bands ranging from 100bp to 20kb.

Pack size: 100 preps

GV200Ambi Clean Gel/PCR DNA Extraction Kit

AmbiClean Kit (Gel & PCR) is a system designed for DNA recovery from agorose gel and rapid PCR clean-up of DNA bands ranging from 100bp to 20kb.

Pack size: 200 preps

Pricelist

Datasheet AmbiClean Purification Mini-Prep Kit

Stability Test Report

MSDS AmbiClean Extraction Mini-Prep Kit

References

Said, M.B., Belkahia, H., Mabrouk,N.E., Saidani, M., Hassen, M.B., Alberti, A., Zobba, R., Bouattour, S., Bouattour, A., Messadi, L. (2017) Molecular typing and diagnosis of Anaplasma spp. closely related to Anaplasma phagocytophilum in ruminants from Tunisia. Ticks and Tick-borne Diseases.8. Pp..412-422

Said, M.B., Belhakia, H., Mabrouk, N.E., Saidani, M., Alberti ,A., Zobba,R., Cherif, A., Mahjoub, T., Bouattour, A., Messadi, L. (2017) Anaplasma platys-like strains in ruminants from Tunisia. Infection, Genetics and Evolution 49. Pp..226-233.

Azwai, S.M. et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya Open Veterinary Journal, 6(1), p. 36-43.

Hamidinejat, H., et al. (2015) Development of an Indirect ELIS using Different Fragments of Recombinant Ncgra 7 for Detection of Neospora caninum Infection in Cattle and Water Buffalo. OIran Journal of Parasitology. 10(1), p.69-77.

Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015) Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.

Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490

Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014) ) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410.