Sera thawing and freezing

Thawing

1. Thaw until a thin liquid layer appears

At room temperature

In a water bath at +15 – +20 °C

2. Following time for warming up to +20 °C in the water bath

3. Following time for warming up to +37 °C in the water bat

The bottles should be immersed in the water bath up to the serum level in the bottle. During warming, the content should be mixed by rotating slowly (do not shake to prevent formation of foam).

Re-freezing of sera

Serum should be frozen quickly, a pre-cooling at +2 – +8 °C is recommended. Frequent freezing and thawing cycles have to be avoided: preferably aliquote after first thawing.

Sera freezing duration

Without pre-cooling (initial temperature approx. +20 °C)

With pre-cooling to +4 °C

Further Information

Heat inactivation
Sera are undefined natural products. as such, in addition to containing growth-promoting substances they can also contain undesirable components (e.g. complement system).

In order to reduce the content of interfering substances, many experimental procedures stipulate inactivation of the serum prior to use, using the so-called “heat inactivation” process. the serum is normally heated to +56 °c for 30 minutes.

Request for samples to test new batches
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