Taq DNA Polymerase blue ready-to-load

Maximo Tag-Blue Polymerase is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed!

Features:
Maximo Taq-Blue DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates.  After PCR reaction the enzyme can be loaded to agarose gel, no dye and DNA loading buffer is needed. The enzyme is time- and cost saving, because it includes dye and loading buffer, already.

Applications:
– Standard / General PCR with visible control
– High-throughput PCR
– Primer extension
– Gene mutation
– T/A cloning

Description:
Maximo Tag-Blue DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  1 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP 40, 0.5% Tween 20, in blue loading buffer

Reaction Buffers supplied with the enzyme:

10X Buffer I:  500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂
MgCl₂: 100 mM

Transportation: on blue ice

Storage: at -20°C for 12 months

Quality control: 
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 2 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination

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