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Real-time-PCR Master Mix E4

The Master mix for qPCR contains: EvaGreen, dUTP. UNG and ROX (500 nM)

– The Master mix contains dUTP instead of dTTP
– The Master contains UNG (Uracil-N-Glycosylase)
– The Mix contains ROX (500nM or LOW-ROX-version 100nM) as passive Reference dye (it provides a baseline in multiplex reactions)
– It contains EvaGreen as fluorescent dye
– The qPCR / RTD-PCR Master mix E4 is ready-to-use and is optimized for high specificity and sensitivity because of optimized reaction buffer
– easy to use because ready-to-use Master Mix

– Detection and quantification of DNA and cDNA targets
– Profiling gene expression
– Microbial detection
– Viral load determination

500 nM Version: ABI: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus
100 nM Version: 7500 System, Stratagene Mx35005P, Mx4000 and Mx3000P, ViiA 7, QuantStudio

The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, EvaGreen, ROX, UNG, optimized reaction buffer with KCl and MgCl2, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000


S9180Real-time-PCR Master Mix E4

100 rcs (2,5 ml)

S9180LReal-time-PCR Master Mix E4

500 rcs (12,5 ml)

S9180XLReal-time-PCR Master Mix E4

1000 rcs (25 ml)





Stability Test Report

Material Safety Datasheet