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Real-time-PCR Master Mix E1

qPCR Master mix with EvaGreen and dUTP

– The Master mix contains dUTP instead of dTTP
– It contains EvaGreen as fluorescent dye
– The qPCR / RT-PCR Mastermix E1 is ready-to-use and is optimized for high specificity and sensitivity because of optimized reaction buffer
– easy to use because ready-to-use Master Mix
– The Master Mix can be used with ROX as reference dye (1x concentrated)

– Detection and quantification of DNA and cDNA targets
– Profiling gene expression
– Microbial detection
– Viral load determination

BioRad: iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384, MJ Opticon, Option2, Chromo4, MiniOpticon
Qiagen: Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000
Eppendorf: Mastercycler realplex
Illumina: Eco RealTime PCR System
Cepheid: SmartCyler
Roche: LIghtCycler 480, LightCycler 2.0

The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix contains dUTP instead of dTTP and allows an UNG (Uracil-N-Glycosylase) treatment at the onset of thermal cycling to prevent carry-over contaminations of DNA from previous PCR reactions.

Concentration: The Master mix is 2x concentrated

List of components:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, EvaGreen, optimized reaction buffer with KCl and MgCl2, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000


S9150Real-time-PCR Master Mix E1

100 rcs (2,5 ml)

S9150LReal-time-PCR Master Mix E1

500 rcs (12,5 ml)

S9150XLReal-time-PCR Master Mix E1

1000 rcs (25 ml)



Stability Test Report

Material Safety Datasheet