qPCR/real-time-PCR Master Mix DLP3 / DLP5

Features:
– The Master mix contains dUTP instead of dTTP
– The 2X Mix contains ROX (500nM, 100nM for LOW-ROX) as passive
Reference dye (it provides a baseline in multiplex reactions)
– The qPCR / RTD-PCR Master mix DLP3/DLP5 is ready-to-use and is
optimized for high specificity and sensitivity because of optimized
reaction buffer
– easy to use because ready-to-use Master Mix

Applications:
– Detection and quantification of DNA and cDNA targets
– Profiling gene expression
– Microbial detection
– Viral load determination

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, ROX, optimized reaction buffer with KCl and MgCl₂, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light