Protein Marker PS10 PLUS

Applications:
– Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
– Monitoring of protein transfer onto membranes during Western blotting.
– Sizing of proteins on SDS-polyacrylamide gels and Western blots.

Usage:
Mini gel application: 10 μl/well; 5 μl per well blots Standard gel application: 20 μl/well; 10 μl per well for blots

Number of bands: 10                               11, 17, 25, 35, 48, 63, 75, 100,135, 180 kDa

Loading:
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
– Thaw marker at room temperature or heat at 37 –40 °C for a few minutes. Do not boil!
– Vortex gently to ensure the solution is homogeneous and load the ladder on SDS-polyacrylamide gel
– 10 μl per well for mini-gels, 5 μl per well for blots
– 20 μl per well for large gels, 10 μl per well for blots

1 ml marker is sufficient for 100 mini gels or 50 standard gels. It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.

Note:
Protein Marker PS10 is optimized for runs on 12 % SDS polyacrylamide gels. 8 to 10 % gels may cause proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.

Quality Control: Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage: at -20°C

Shipment: on blue ice

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