Protein marker/ Protein ladder EXtended PS 13 (5 – 245 kDa)

Applications:
– Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
– Monitoring of protein transfer onto membranes during Western blotting.
– Sizing of proteins on SDS-polyacrylamide gels and Western blots.

Number of bands: 13      5, 11, 27, 20, 25, 35, 48, 63, 75, 100, 135, 180, 245 kDa

Loading: 
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
– Thaw marker at room temperature or heat at 37 –   40 °C for a few minutes. Do not boil!
– Vortex gently to ensure the solution is homogeneous and load the ladder on SDS-polyacrylamide gel
– 5 μl per well for mini-gels, 2.5 μl per well for blots
– 10 μl per well for large gels, 5 μl per well for blots

1 ml marker is sufficient for 200 mini gels or 100 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.

It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.

Usage:
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots

Shipment: on blue ice

Storage: at -20°C or at room temperature

Quality Control: Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Note:
Protein Marker PS11 is optimized for runs on 15 % SDS polyacrylamide gels. 4 to 12 % gels may cause
proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.

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