Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Pfu/Psp DNA polymerase replicates DNA at 75°C catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of Mg+. Pfu DNA polymerase possesses 3′ to 5′ exonuclease proof reading activity that enables the polymerase to correct nucleotide-misincorporation errors. To reduce the risk of contamination, pipetting errors and to increase the repeatable of results the 2X-preMix contains an optimized mixture of enzyme, dNTP’s and reaction buffer. Just add your template DNA and primers.
– blunt end PCR cloning
– PCR and primer extension where “high fidelity” is required
– Site-directed mutagenesis
– PCR where visual control is needed
Pfu/Psp DNA polymerase 2X-preMix is isolated from the archae bacteria Pyrococcus f-species, a thermostable Polymerase of approximately 90000 daltons. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. The Pfu/Psp DNA Polymerase has no detectable reverse transcriptase activity.
Concentration: Premix 2X (25µl per reaction)
One unit is defined as the amount of enzyme required to catalyze the incorporationof 10 nM of dNTPs into acid insoluble material in 30 minutes at 75°C.
Storage: at -20°C for 24 months
Transportation: on blue ice
Stability tests / Quality Tests
– Tested for the DNA amplification of 2,2 kb from lambda DNA
– Contamination level check of bacterial DNA
– Purity by SDS-Page > 90 %
|S9121||Pfu/Psp DNA Polymerase 2X Pre-Mix ready-to-use (2×1,25 ml)|
|S9122||Pfu/Psp DNA Polymerase 2X Pre-Mix ready-to-use (2×1,25 ml)|
5 x 100 rcs
Stability Test Report
Material Safety Datasheet