Pfu/Psp DNA Polymerase 2X Pre-Mix (ready-to-use)

Features:
Pfu/Psp DNA polymerase replicates DNA at 75°C catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of Mg⁺. Pfu DNA polymerase possesses 3′ to 5′ exonuclease proof reading activity that enables the polymerase to correct nucleotide-misincorporation errors. To reduce the risk of contamination, pipetting errors and to increase the repeatable of results the 2X-preMix contains an optimized mixture of enzyme, dNTP’s and reaction buffer. Just add your template DNA and primers.

Applications:
– blunt end PCR cloning
– PCR and primer extension where “high fidelity” is required
– Site-directed mutagenesis
– PCR where visual control is needed

Description:
Pfu/Psp DNA polymerase 2X-preMix is isolated from the archae bacteria Pyrococcus f-species, a thermostable Polymerase of approximately 90000 daltons.  Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. The Pfu/Psp DNA Polymerase has no detectable reverse transcriptase activity.

Concentration: Premix 2X (25µl per reaction)

Unit definition:
One unit is defined as the amount of enzyme required to catalyze the incorporationof 10 nM of dNTPs into acid insoluble material in 30 minutes at 75°C.

Storage: at -20°C for 24 months

Transportation: on blue ice

Stability tests / Quality Tests

– Tested for the DNA amplification of 2,2 kb from lambda DNA
– Contamination level check of bacterial DNA
– Purity by SDS-Page > 90 %