One.Step RT-PCR Probe Kit

The MaximoOne.Step RT-qPCR Kit is designed for quantitative real-time analyses of RNA templates using Dual Labeled Fluorescent Probes

Features:

• The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers and the dual labeled fluorescent probe.
• The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts

Description:
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs and reaction enhancers. The basis is a Real-time PCR with dual labeled probes and multiplexing capacity.

Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

Components  of Maximo.OneStep RT-qPCR (for probes):
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs
RNase-free water

Storage: at -20°C, avoid frequent thawing and freezing

Transport: the product will be shipped with “blue ice”

For optimal results it is recommended to make an individually optimization for each RNA / primer pair.

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000

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