One.Step RT-PCR Master Mix with EvaGreen/ROX

Great Mastermix for RT-realtime PCR based on a genetically engineered reverse transcriptase; for improved efficiency, thermostability and specivicity; structured and long cDNA fragments; contains EvaGreen as intercalating dye and ROX as reference

• The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That’s all.
• The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts

Description:
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs, EvaGreen as intercalating dye; ROX and reaction enhancers.

Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. The kit is optimized for all real-time PCR cyclers who are compatible with the evaluation of the ROX reference signal. Select the same optical setting for EvaGreen than for SYBR-Green EvaGreen (exc_max= 500 nm; Emi_max= 530 nm).

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler.

Storage: @ -20°C, avoid frequent thawing and freezing, store all components with EvaGreen / ROX in the dark

Transport: the product will be shipped with “blue ice”

For optimal results it is recommended to make an individually optimization for each RNA / primer pair.

Components of Maximo.OneStep RT-qPCR Mix:

Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA dye and ROX

RNase-free water

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000

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