The superior Mastermix is based on a genetically engineered reverse transcriptase; for improved efficiency; structured and long cDNA fragments; contains EvaGreen as intercalating dye
- The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That’s all
- The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs, EvaGreen as intercalating dye and reaction enhancers.
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template.
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler
Components of Maximo.OneStep RT-qPCR:
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA and stabilizers
at -20°C, avoid frequent thawing and freezing, store all components with EvaGreen in the dark
the product will be shipped with “blue ice”
Stability tests / Quality control / Comparison
Manufactured and quality-controlled in accordance with ISO 9001:2000
|1905-520||One.Step RT-PCR Master Mix with EvaGreen|
2 x 1,25 ml
|1905-522||One.Step RT-PCR Master Mix with EvaGreen|
10 x 1,25 ml