One.Direct.Step RT-qPCR Kit for Probes, DLP-Line

Direct PCR: Amplification directly from blood or cell material – Safe time, increase your result

Performance:
The RT-qPCR kit ensures fast and easy preparation with a minimum of pipetting steps and is highly recommended for:

– direct detection of RNA viral pathogens in various tissues
– direct amplification of target RNA from sample materials
– point-of-care Diagnostics

Description:
The kit is recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes but can also be used without fluorescent probes in standard PCR assays. The kit contains an enzyme mixture including a genetically engineered reverse transcriptase and an antibody-inhibited Taq polymerase. The 2x conc. reaction mix contains ultrapure dNTPs and an unique buffer system optimized to resist various PCR inhibitors in unpurified sample material

One.Direct.Step RT-qPCR for Probes is designed for quantitative real-time analysis of target RNA directly from whole blood, swabs and animal- or plant tissue without the requirement of any prior RNA purification steps

shipping and storage: transportation with blue ice; storage @ -20°C for at least 16 months (stable @ +4°C up to 4 weeks), avoid frequently freeze/thaw cycles

Kit content:
Extraction Buffer: 10x concentrated
Direct Enzyme: Mix of engineered reverse transcriptase, antibody-inhibited hot start polymerase and RNase inhibitor in storage buffer with 50 % glycerol (v/v)

Direct Reaction Mix: 2x conc. buffer system containing dNTPs, enhancer and stabilizer
PBS (phosphate buffered saline): 10x concentrated
PCR-grade Water

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000

Pricelist