Maximo Taq DNA Polymerase

Maximo Taq Polymerase is a highly purified polymerase for routine amplification

Features:
Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.

Applications:
– Standard / General PCR
– Multiplex PCR
– High-throughput PCR
– Primer extension
– Gene mutation
– T/A cloning

Description:
Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20

Reaction Buffers supplied with the enzyme:

10X Buffer I:  500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂

Quality control:
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 3 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination

Transportation: on blue ice

Storage: at -20°C for 24 months

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