Maximo Taq DNA Polymerase 2X-preMix / PCR Master Mix

Maximo Tag Polymerase PCR-Master-mix as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR

Features:
Maximo Taq DNA Polymerase 2X-preMix provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit. The optimized mixture of all components reduces pipetting mistakes and ensures repeatable results – every day.

Applications for PCR Mastermix:
– Standard / General PCR
– optimized for high specifity
– High-throughput PCR, automated pipetting, or plate based PCR
– Gene mutation
– T/A cloning

Description:
Maximo Taq DNA Polymerase 2X-Mastermix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template has to be added.
Maximo Taq DNA Polymerase 2X-preMix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration: the mixture is 2X concentrated (Mg2+: 3,6 mM, 1,8 mM final)

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

List of components:
0.1U/ul Taq DNA Polymerase, 0.4 mM dATP, 0.4 mM dGTP, 0.4 mM dCTP, 0.4 mM dTTP, 4 mM MgSO₄, 20 mM KCl, 16 mM (NH₄)₂SO₄, 20 mM Tris-HCl, pH8.8

Quality control:
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 2 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination

Transportation: on blue ice

Storage: at -20°C for 24 months

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