Maximo Tag Polymerase PCR-Master-mix as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR
Maximo Taq DNA Polymerase 2X-preMix provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit. The optimized mixture of all components reduces pipetting mistakes and ensures repeatable results – every day.
Applications for PCR Mastermix:
– Standard / General PCR
– optimized for high specifity
– High-throughput PCR, automated pipetting, or plate based PCR
– Gene mutation
– T/A cloning
Maximo Taq DNA Polymerase 2X-Mastermix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template has to be added.
Maximo Taq DNA Polymerase 2X-preMix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.
Concentration: the mixture is 2X concentrated (Mg2+: 3,6 mM, 1,8 mM final)
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree
List of components:
0.1U/ul Taq DNA Polymerase, 0.4 mM dATP, 0.4 mM dGTP, 0.4 mM dCTP, 0.4 mM dTTP, 4 mM MgSO4, 20 mM KCl, 16 mM (NH4)2SO4, 20 mM Tris-HCl, pH8.8
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 2 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination
Transportation: on blue ice
Storage: at -20°C for 24 months
|S9113||PCR Mastermix / Taq DNA Polymerase Premix 2X (4×1,25 ml)|
2 x 100 rcs
|S9114||PCR Mastermix / Taq DNA Polymerase Premix 2X (4×1,25 ml)|
10 x 100 rcs