First Strand cDNA PLUS Synthesis Kit

The Maximo First Strand cDNA Synthesis Set includes all components for a simple and flexible first strand cDNA synthesis

Description
The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.

Features:
– for synthesis of cDNA from 100 bp up to 10 kb length
– higher cDNA yield and greater efficiency for full length cDNA synthesis compared with standard M-MLV RT
– increased thermostability

Applications:
– Synthesis of highly structured and long cDNAfragments
– extremely sensitive and highly specific RTPCR
– DNA labeling

Components:
– Reverse Transcriptase (200 U / µl)
– 5 X Complete RT Buffer
– dNTP Mix (10 mM each)
– DTT stock solution (100 mM)
– Oligo-(dT)₂₀ primer (100 µM)
– Random Hexamers (100 µM)
– RNase Inhibitor (40 U / µl)
– RNase-free Water

QC-Tests:
– Purified free of endo- and exodeoxyribonucleases, phosphatases and
ribonuclease
– Activity and stability tested in first strand cDNA synthesis

Storage: Store product at -20°C. Avoid frequent thawing and freezing

Transport: Product will be send with “blue ice”

Manufactured and quality-controlled in accordance with ISO 9001:2000

Protocols:
Note:
For many standard combinations of RNA and primers heat treatment may be omitted with no negative effect on results. A sample denaturation is particularly recommended for RNA targets that:
– exhibit a high degree of secondary structure
– self- or cross-complementary primers
– initial experiments with new targets

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