DNA Marker 1000 bp / 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis.
Description:
The 1000 bp/1 kb DNA Ladder has a number of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 1000 bp/1 kb DNA Ladder was designed for precise qualitative and approximately quantification of DNA mass. There are no unspecific bands besides the fragments. The 500 bp, 1000 bp and 3000 bp fragment are used as reference band.
Features:
1000 bp/1 kb DNA Marker from GeneON is composed of 13 individual DNA fragments which are re-dissolved in storage buffer
Usage: 1 µg/lane
Concentration: 0,2 µg/µl
Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl
Tracking Dye: 6X DNA Loading dye required
Number of bands: 13 fragments
Fragmente und ungefähre DNA Masse:
base pairs | 250 | 500 | 750 | 1000 | 1500 | 2000 | 2500 | 3000 | 4000 | 5000 | 6000 |
~ DNA mass (ng) | 20 | 30 | 60 | 210 | 50 | 60 | 70 | 200 | 60 | 60 | 60 |
Lademenge: 0.5 – max. 1.2 µg (2-6 µl) pro Spur in 1.7 – 2.5 % Agarose. Wir empfehlen die Verwendung von 1/10 des Ladepuffers im Verhältnis zur DNA Leiter.
Transport: Kühlakkus oder Raumtemperatur
Lagerung: bei -20°C für 24 Monate
Product | Description |
3905-005 | DNA Ladder 1000 bp / 1 kb DNA Marker 1000 bp / 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis. 50 µg – 500 µl |
3905-025 | DNA Ladder 1000 bp / 1 kb DNA Marker 1000 bp / 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis. 5 x 50 µg – 500 µl |