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DNA-Loading dye 6X

6-fold concentrated loading dye for Agarose and Acrylamide gels

The loading dye increases the density of the sample and they add colour to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.

In 1% agarose gels, bromophenol blue migrates with 300 bp linear double-stranded DNA fragment, whereas xylene cyanol FF migrates at approximately the same rate as linear double-stranded DNA 4 kb length. These relationships are not significantly affected by the concentration (0.5 to 1.4%) of agarose in the gel.

Loading: The loading dye suits well for the DNA samples dissolved either in water or in EDTA-containing buffer (as TE buffer). If DNA markers are not prediluted with the Loading dye solution, then mix: The loading buffer is 6x concentrated, that means you have to use it 5:1.

For DNA markers, apply 0.1 µg per 1 mm of agarose gel lane width. Often 1µg of marker is used in one electrophoresis run but it depends on the size of your gel and the comb.

Preparation: 6x Loading Dye Solution deionised water at a ratio 1:1:4 for example, 5 µl 1 kb ladder : 5 µl 6x loading dye : 20 µl water. By applying 30.0 µl of this mixture, you’ll have 1.0 µg of total DNA per lane.

Storage and transport: at RT or -20 °C


3906-205DNA-Loading dye 6X – 5×1 ml

6-fold concentrated loading dye for Agarose and Acrylamide gels

5 x 1 ml




Datasheet DNA Loading-buffer 6X

Stability Test Report

Material Safety Datasheet