Description:
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. (Molecular Biology Grade)
| Synonym | 4′,6-Diamidino-2-phenylindole dihydrochloride |
| State of matter | Solid |
| Melting point | approx. 330°C |
| Formula | C16H15N5 · 2HCl |
| M | 350.25 g/mol |
| CAS-No.: | 28718-90-3 |
| EC-No.: | 249-186-7 |
| Storage: | 2-8°C, protected from light |
| LGK: | 10 – 13 |
| WGK: | 1 |
| Specification | |
| DNases/RNases/Proteases | not detectable |
| Assay (from N) | min. 98 % |
| Solubility (1 %; H2O) | clear |
| UV spectrum | complies to standard |
| Product | Description |
| CE9117 | DAPI 5 g |
| CE9118 | DAPI 25 g |
| CE9119 | DAPI 100 g |
Datasheet
Stability Test Report
Material Safety Datasheet
References
(1)A simple cytochemical technique for demonstration of DNA in celles infected with mycoplasmas and viruses.
(Russel, W.C. et al. (1975) Nature 253, 461-462)
(2)Interaction of DAPI with synthetic polynucleotides. (Kapuscinski, J. & Szer, W. (1979) Nucleic Acids Res. 6,
3519-3534)
(3)Binding of DAPI to GC and Mixed Sequences in DNA: Intercalation of a Classical Groove-Binding Molecule.
(Wilson, W.D. et al. (1989) J. Am. Chem. Soc. 111, 5008-5010)
(4)DAPI Staining of Fixed Cells for High-Resolution Flow Cytometry of Nuclear DNA. (Otto, F. (1990) Methods
Cell Biol. 33, 105-110)
(5)Reverse Fluorescent Chromosome Banding with Chromomycin and DAPI. (Schweizer, D. (1976)
Chromosoma 58, 307-324)
(6)Quantitative Fluorescent Analysis of Different Conformational Forms of DNA Bound to the Dye DAPI, and
Separated by Gel Electrophoresis. (Naimski, P. et al. (1980) Anal. Biochem. 106, 471-475)
(7)Spectrofluorometry of Dyes with DNAs of Different Base Composition and Conformation. (Daxhelet, G.A. et
al. (1989) Anal. Biochem. 179, 401-403)
(8)Detection of Mycoplasma Infection of Mammalian Cells. (Xia, H. et al. (1997) BioTechniques 22, 934-936)