BCIP
Description:
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. (Molecular Biology Grade)
| Synonym | 5-Bromo-4-Chloro-3-Indolyl Phosphate p-Toluidine Salt, X-Phos p-Toluidine Salt, X-Phos-pT |
| State of matter | Solid |
| Solubility (20°C) | 20 mg/mL (DMF) |
| Formula | C8H6BrClNO4P · C7H9N |
| M | 433.64 g/mol |
| CAS-No.: | 6578-06-9 |
| EC-No.: | 229-506-1 |
| Storage: | -20°C, protected from light |
| Hazard pictogram(s) |  |
| Hazard statement(s) | H315-H319-H335 |
| Precautionary statement(s) | P305+P351+P338 |
| Signal word | Warning |
| WGK: | 2 |
| Specification | |
| Assay (HPLC) | min. 98 % |
| λmax. (buffer pH 7.0) | 286 – 294 nm |
| Water (K.F.) | max. 1 % |
| Product | Description |
| CE9108 | BCIP 250 mg |
| CE9109 | BCIP 1 g |
Datasheet
Stability Test Report
Material Safety Datasheet
References
(1) Horwitz, J.P. et al. (1966) J. Med. Chem. 9, 447
Substrate for cytohistochemical determination of enzyme activities: Dihalo-3-indolylphosphates.
(2) Gossrau, R. (1978) Histochemistry 58, 203-218
Comparison of the sensitivity of different hydrolase substrates for their histochemical determination.
(3) Leary, J.J. et al. (1983) Proc. Natl. Acad. Sci. USA 80, 4045-4049
Bio-blots for DNA and RNA with BCIP as a substrate/phosphatase linked antibody.
(4) Ey, P.L. & Ashman, L.K. (1986) Methods Enzymol. 121, 497-509
Determination of alkaline phosphatase connected antibodies in immunoblots.
(5) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Supplement 66, Page 10.8.17. Greene Publishing & Wiley-Interscience, New York.