Isolierung/Aufreinigung von DNA aus Agarosegel
Beschreibung:
Das GF-1 Gel extraction Kit ist ein System für die einfache und zeitsparende Isolierung von DNA mit einer Fragmentgröße von 100bp bis 10kb aus Agarosegel in TAE-Puffer (Tris-Acetat/EDTA) oder TBE-Puffer (Tris-Borat/EDTA).
Spezielle im Kit enthaltene Puffer optimieren die Bindung der DNA auf eine spezielle Glasfilter-Membran zur effizienten Gewinnung von hochreiner DNA.
Die hochreine DNA kann für alle Arten von Folgeexperimenten in der Molekularbiologie, wie zum Beispiel Restriktionsenzymverdau, radioaktive/fluoreszierende DNA-Sequenzierung, PCR, Ligation und Transformation weiterverwendet werden.
Features:
– Hohe Effizienz von 90%
– Minicolumn Spin-Technologie
– Hohe Zeitersparnis, Aufreinigung in weniger als 20 Minuten möglich
Anwendung: Zur Reinigung und Gewinnung von DNA aus Agarosegelen.
Kit Bestandteile:
– GF-1 columns
– Collection tubes
– Gel DNA Binding Puffer (Puffer GB)
– Waschpuffer (Konzentrat)*
– Elutionspuffer
– Handbuch
* Bitte beachten Sie Verdünnung von Lösungen und Lagerung und Stabilität vor der Verwendung dieses Kits.
Artikel | Beschreibung |
GP05 | Gel Extraction Kit The Gel Extraction Kit is a system designed for rapid purification of DNA bands ranging from 100bp to 10kb from all grades of agarose gel in TAE (Tris-acetate/ EDTA) or TBE (Tris-borate/ EDTA) buffer. St./Karton: 5 preps |
GP50 | Gel Extraction Kit The Gel Extraction Kit is a system designed for rapid purification of DNA bands ranging from 100bp to 10kb from all grades of agarose gel in TAE (Tris-acetate/ EDTA) or TBE (Tris-borate/ EDTA) buffer. St./Karton: 50 preps |
GP100 | Gel Extraction Kit The Gel Extraction Kit is a system designed for rapid purification of DNA bands ranging from 100bp to 10kb from all grades of agarose gel in TAE (Tris-acetate/ EDTA) or TBE (Tris-borate/ EDTA) buffer. St./Karton: 100 preps |
GP200 | Gel Extraction Kit The Gel Extraction Kit is a system designed for rapid purification of DNA bands ranging from 100bp to 10kb from all grades of agarose gel in TAE (Tris-acetate/ EDTA) or TBE (Tris-borate/ EDTA) buffer. St./Karton: 200 preps |
Stability Test Report
MSDS Gel Extraction Mini-Prep Kit
References
Mojarad, M., Alemzadeh, A., Ghoreishi, G., Javaheri, M. (2016) Kerosene biodegradation ability and characterization of bacteria isolated from oil-polluted soil and water. Journal of environmental Chemical Engineering.
Nasiri, V., Teymurzadeh, S., Karimi, G., Nasiri, M. (2016). Molecular detection of Toxoplasma gondii in snakes. Experimental Parasitology. 169. Pp102-106.
Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38
Erdogmus, S.F., et al (2015) Hydrocarbon Utilization Ability of Chromohalobacter sp. Ekoloji, 94: 10-16.
Noikong, W., Wongsawad, C. (2014) ) Epidemiology and molecular genotyping of echinostome metacercariae in Filopaludina snails in Lamphun Province, Thailand. Asian Pacific journal of tropical Medicine. 7(1). Pp.26-29.
Sagri, E., et al. (2014) Olive Fly Transcriptomics Analysis Implicates Energy Metabolism Genes in Spinosad Resistance. BMC Genomics. 15(714), p.1-20.
Bondoc, O.L., Dominguez, J.M.D., & Peñalba, F.F. (2013) DNA Barcoding of Domestic Swine Breeds and Crossbreeds (Sus scrofa) in the Philippines. Philippine Journal of Veterinary and Animal Sciences39(1): 31-42.
Farnia, P., Bandehpour, M., Ghanavi, J., & Kazemi, B. (2013) Cloning and Expression of Soluble Vascular Endothelial Growth Factors Receptor-1 (sFlt-1) Fragments in CHO-K1. International Journal of Clinical and Experimental Medicine, 6(9): 773–778.
Hussain, A. and Idrees, M. (2013) The First Complete Genome Sequence of HCV-1a from Pakistan and a Phylogenetic Analysis with Complete Genomes from the Rest of the World. Virol J.; 10: 211.
Bondoc, O.L., & Santiago, R.C. (2012)The Use of DNA Barcodes in the Evolutionary Analysis of Domestic Breeds and Strains of Chicken (Gallus gallus domesticus) in the Philippines The Philippine Agricultural Scientist, 95(4): 358-369.
Gharajelar, S.N., Ahmadi, M., & Hosseini, B. (2012) Cloning and Expression of the Immunogenic Moiety of Pseudomonas aeruginosa Exotoxin A. Biological Journal of Microorganism1(4): 7-14.
Gul, R., et al. (2012)Expression and Sequence Characterization of Growth Hormone Binding Protein of Nili-Ravi Buffaloes (Bubalus bubalis) African Journal of BiotechnologyProQuest. 11(57), p. 12103-12109
Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011) Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114.
Heng, J.L.S., et al (2011)Isolation, Characterization and Identification of Potential Actinbobacteria with Antifungal Activities towards Chilli Anthracnose African Journal of Biotechnology. ProQuest10(32), p. 5979-5987.
Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010) Isolation and Characterization of a Novel Thermophilic-organic Solvent Stable Lipase from Acinetobacter baylyi. Applied Biochemistry and Biotechnology.