Für die Visualisierung und Quantifizierung von Proteinen, die durch 1-D oder 2D SDS /PA Gele
Fluorescent-Protein-Gel-Juice ist ein Fluoreszenzfarbstoff zur Visualisierung und Quantifizierung von Proteinen, die durch 1-D oder 2D SDS – PA-Gelelektrophorese getrennt wurden. Durch die Anlagerung von Fluorescent-Protein-Gel-Juice an Proteine entsteht eine starke Fluoreszenz.
Die Färbung mit Fluorescent-Protein-Gel-Juice erfolgt in zwei Schritten und kann daher in weniger als 30 Minuten abgeschlossen werden. Zunächst werden die Gele mit Ethanol / Essigsäure-Lösung fixiert und anschließend mit Fluorescent-Protein-Gel-Juice Lösung gefärbt. Ein Entfärbungsschritt wird normalerweise nicht empfohlen, kann aber durchgeführt werden, um Hintergrund zu reduzieren. Hierzu wird das Gel in Wasser ca. 5 Minuten gemischt. Die mit Fluorescent-Protein-Gel-Juice gefärbten Gele können direkt mit UV-induzierte Fluoreszenz Bild-Systemen visualisiert werden. Die maximale Emissions-Wellenlänge von Nimble-juice gebundenen Proteine liegt bei ca. 570 nm.
Der gebundene Fluorescent-Protein-Gel-Juice Farbstoff kann durch Eintauchen des Gels in ausreichend Wasser sehr leicht aus Proteinen entfernt werden, so dass die Aufarbeitung, z. B. für eine nachfolgenden enzymatischen Verdauung, ermöglicht wird. Gefärbte Gele können in Färbelösung im Dunkeln bei 2-8 ° C gelagert werden.
| Artikel | Beschreibung |
| 3910012 | Fluorescent-Protein-Gel-Juice Maximo-Gel fluorescent protein gel stain (100x) is exceptionally fast and sensitive. Sensitivity of Maximo-Gel juice is of the same general order as silver stain or other fluorescent protein stains 10 ml |
| 9310012L | Fluorescent-Protein-Gel-Juice Maximo-Gel fluorescent protein gel stain (100x) is exceptionally fast and sensitive. Sensitivity of Maximo-Gel juice is of the same general order as silver stain or other fluorescent protein stains 10 ml |
Datasheet Fluorescent-Protein-Gel-Juice
Stability Test Report
Material Safety Datasheet
Instructions for Use of Maximo-Gel juice
Prepare Fix Solution
The fix solution consists of 40% (v/v) ethanol* and 7% (v/v) acetic acid. Prepare the solution with distilled or deionized water that has been filtered. The quantity of fix solution required depends on the number of gels to be stained and the size of the gel as indicated in the table below. As a general guide, use an amount of fix solution equal to 10-15 times the volume of the gel.
| Gel Size | Volume of Fix Solution per Gel |
| ~9 cm × 7 cm (general mini-gel) | ~ 100 ml |
| ~ 3 cm × 9cm | ~ 200 ml |
| ~ 16cm × 16cm | ~ 500 ml |
| ~ 26 cm × 23 cm | ~ 1,000 ml |
* 40% ethanol can be replaced with 50% methanol, but methanol is not recommended in consideration of safety and stain performance.
Fix Gels
Remove the gel(s) from the gel cassette or plates. Place gel in a clean glass or plastic tray with the volume of fix solution indicated above. Cover the tray, place on a rocker or shaker and agitate gently.
For standard protocol, fix at room temperature for 60 minutes.
For quick fixing, microwave the fix solution with the gel to boil (~1.5 minutes for 100 ml of fixing solution; dependent on volume and microwave power), and then agitate gently at room temperature for 15 minutes.
Note: Either shortened or prolonged fix time may reduce sensitivity-
Prepare Working (1x) Stain Solution
Working (1x) stain solution is prepared by diluting one volume of Maximo-Gel juicewith 100 volumes of distilled or deionized water that has been filtered. The quantity of stain solution required depends on the number of gels to be stained and the size of the gel as indicated in the table below. As a general guide, use an amount of working stain solution equal to 10 times the volume of the gel.
| Gel-size | Volume of Stain Solution |
| ~ 9 cm × 7 cm | ~ 60 ml |
| ~ 13 cm × 9 cm | ~ 120 ml |
| ~ 16 cm x 16 cm | ~ 300 ml |
| ~ 26 cm × 23 cm | ~ 600 ml |
Working (1x) stain solution is recommended to be freshly prepared prior to its intended use.
Stain Gels:
Carefully pour off the fix solution and add Maximo-Gel juice (1x working solution) to the staining tray. Cover the tray, place on a rocker or shaker and agitate gently at room temperature. For standard protocol, stain for at least 45 minutes For rapid analysis, stain for 15-45 minute.
Generally, proteins (5 ng or below) might be detectable after 15 minutes of staining.
Gels may be left in the Maximo-Gel juice solution for extended periods, with care to limit light exposure. Stained gels may be stored for up to three months without significant loss of fluorescent signals. For long-term storage, gels should be placed in sealable plastic bags with 5–10 ml of stain solution and stored in the dark at 2–8 °C.
NOTE: Destaining is not necessary. However, it might be helpful to remove excess dye from the gel surface by quickly rinsing the gel with clean water. Staining intensity persists or even increases when the gel is stored in Maximo-Gel juice solution at 4 °C.
Gel Imaging
Gels stained with Maximo-Gel juice are visualized using UV light excitation. We recommend to adjust excitation/emission to around 330nm/570nm for best results. If the imaging equipment has no preprogrammed imaging function for Maximo-Gel juice, the imaging setting for SYPRO Ruby stain or Ethidium bromide that uses UV transillumination is recommended. Any imaging system using UV light excitation may be used to image Maximo-Gel juice.