BSA zur Stabilisierung von Enzymen
Konzentration: 10 mg / ml (1 % ) (oder andere Konzentration auf Anfrage)
Lagerpuffer: 20 mM KPO4, 50 mM NaCl, 0.1 mM EDTA, 5% Glyzerin, pH 7.0 @ 25°C
Lagerung: -20°C
Transport: mit Kühlakkus
Qualitätskontrollen:
16-Hour Incubation:
A 50 µl reaction containing 1 µg of FX174 DNA (HaeIII digested) and 50 µg of BSA incubated for 16 hours at 37ºC resulted in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.
RNase Assay:
Incubation of 50 µg of BSA with 1 µg RNA Ladder for 1 hour at 37°C in 50 µl assay buffer [70 mM Tris-HCI, 10 mM MgCl2, 5 mM DTT, (pH 7.6, 25°C)] followed by agarose gel electrophoresis resulted in no detectable RNA degradation.
Endonuclease Assay:
Incubation of 50 µg of BSA with 1 µg FX174 RF I DNA for 4 hours at 37°C in 50 µl of 1X Reactionbuffer 2 resulted in < 10% conversion to RF II.
| Artikel | Beschreibung |
| 2909-005 | Bovine Serum Albumin – BSA BSA prevent the adhesion of enzymes at reaction tubes NEB-Buffer 4 x 1,5 ml |
| 2909-005W | Bovine Serum Albumin – BSA BSA prevent the adhesion of enzymes at reaction tubes water-buffered 4 x 1,5 ml |
Datasheet Bovine Serum Albumin
Stability Test Report
Material Safety Datasheet
