Extraktion hochwertiger Gesamt-DNA aus grampositiven oder gramnegativen Bakterien
Beschreibung:
Das GF-1 Bacterial DNA extraction Kit wird für die Extraktion hochwertiger Gesamt-DNA aus grampositiven oder gramnegativen Bakterien verwendet. Das speziell entwickelte und optimierte Puffersystem erlaubt die Reinigung von bis zu 20μg DNA. Das Kit beinhaltet Columns, die mit einer speziellen Glasfilter-Membran ausgestattet sind, um so die DNA effizient zu binden.
Die bakterielle DNA-Aufbereitung beruht auf dem Prinzip der Minicolumn Spin-Technologie. Zellulärer Debris, Salze und andere Kontaminationen können durch zwei kurze Waschschritte mit speziellen Puffern schnell und effizient entfernt werden. Die gereinigte und qualitativ sehr hochwertige DNA wird abschließend in Elutionspuffer eluiert.
Die Gesamtzell-DNA, die mit dem GF-1 Bacterial DNA extraction Kit isoliert wurde, kann für alle Arten von Folgeexperimenten, wie zum Beispiel PCR, RFLP-Analysen oder Southern Blotting weiterverwendet werden.
Kit Bestandteile:
– Columns
– Collection tubes
– Resuspension Puffer 1
– Resuspension Puffer 2
– Bacterial Genomic Binding Puffer
– Waschpuffer
– Elutionspuffer
– Proteinase K Lösung *
– Handbuch
* Bitte beachten Sie die richtige Lagertemperatur.
Artikel | Beschreibung |
BA05 | Bacterial Genomic Extraction Kit For purification up to 20μg of a high molecular weight genomic DNA from 1-3 ml either Gram-negative or Gram–positive bacteria St./Karton: 5 preps |
BA50 | Bacterial Genomic Extraction Kit For purification up to 20μg of a high molecular weight genomic DNA from 1-3 ml either Gram-negative or Gram–positive bacteria St./Karton: 50 preps |
BA100 | Bacterial Genomic Extraction Kit For purification up to 20μg of a high molecular weight genomic DNA from 1-3 ml either Gram-negative or Gram–positive bacteria St./Karton: 100 preps |
MSDS Bacterial Genomic Extraction Mini-Prep. Kit
Referenzen
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